The mRNA and protein level of ABI5 in overexpression plants on WT and mutant backgrounds were examined by quantitative real‐time PCR and immunoblotting, respectively. Transgenic lines with similar amounts of ABI5 (En‐2 Super:ABI5‐MYC vs bes1‐D Super:ABI5‐MYC ) were selected as a pair, and two independent pairs were selected and used for the following studies (Fig. S2 a,b).

By analogy to the related Novel Interactor of JAZ (NINJA) protein, it was suggested that the AFPs interact with the co-repressor TOPLESS to inhibit ABA-regulated gene expression. 2020-05-08 The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. In sleepy1 mutant seeds, RGL2 overaccumulates; germination of these seeds can occur under conditions that produce low ABI5 … Overexpression of ABI5 in Col-0 influences flowering time To assess the correlation between flowering time and ABI5 expression, overexpressing transgenic lines carrying plas-mid p35S::ABI5-HA in Col-0 background were generated. A total of 43 lines were analysed by examining ABI5-HA protein level in individual transgenic plants (Fig.

Abi5 overexpression

Similar data were also obtained when ABI3 and ABI5 were expressed in the JAZ5-ΔJas background ( Supplemental Figures 12C and 12D ). Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas exchange when plants experience periodic drought. Noteworthily, we showed that down-regulation of NF-YC9 represses, but overexpression of NF-YC9 enhances, ABA-induced ABI5 expression (Fig. 7d), supporting the idea that NF-YC9 both facilitates ABI5 activity and interacts with other components such as RGL2 (Liu et al. 2016) to directly target to and stimulate ABI5 gene expression in response to ABA. 23 Oct 2015 To assess a physiological role for S-nitrosylation of the ABI5 protein during seed germination, ABI5- and ABI5Cys153Ser-overexpressing lines 9 Aug 2019 Overexpression of the soybean (Glycine max) GmbZIP1 gene improved high salt stress tolerance in transgenic plants (Gao et al.

Srinivas S. L. Gampala, Ruth R. Finkelstein, Samuel S. M. Sun, Christopher D. Rock

To emphasize the effects of ABI4 and ABI5 expression, we analyzed 11 day old seedlings, a developmental stage when seed specific transcription factors are not normally expressed but overexpression of ABI4 or ABI5 enhances ABA-inducible responses (Brocard et al. 2002; Söderman et al.

The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. In sleepy1 mutant seeds, RGL2 overaccumulates; germination of these seeds can occur under conditions that produce low ABI5 …

Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell. Overexpression of MdABI5 promoted ABA-induced anthocyanin biosynthesis, which was partially dependent on MdbHLH3. Further studies showed that MdABI5 can not only interact with, and enhance the transcriptional activity of MdbHLH3 to target genes MdDFR and MdUF3GT, but can also directly bind to the promoter of MdbHLH3 and activate its expression. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2.

2020-03-02 · Consistently, PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness, and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark.
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To identify transcriptional regulators involved in ABI5-mediated Cd accumulation, we searched for ABI5-interacting proteins and identified the Cd-induced R2R3-MYB TF MYB49 . Overexpression of ABI5 altered flowering time. (A) The expression level of ABI5-HA fusion protein in individual transgenic lines of p35S::ABI5-HA. Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ- accumulating (JAZ-DJas) plants.

Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas … ABI5 Binding Proteins (AFPs) are a family of negative regulators of bZIP transcription factors of the AREB/ABF family, which promote ABA responses.
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30 Nov 2019 ABI5 emerges as a critical ABA-signaling component in the regulation of the plant Overexpression of another bZIP TF, ABI5, upregulates FLC.

In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYLs is also affected during the germination of abi5 mutants. The mRNA and protein level of ABI5 in overexpression plants on WT and mutant backgrounds were examined by quantitative real‐time PCR and immunoblotting, respectively. Transgenic lines with similar amounts of ABI5 (En‐2 Super:ABI5‐MYC vs bes1‐D Super:ABI5‐MYC ) were selected as a pair, and two independent pairs were selected and used for the following studies (Fig. S2 a,b). Overexpression of RAV1 repressed ABI3, ABI4, and ABI5 expression, and RAV1 bound to the ABI3, ABI4, and ABI5 promoters in vitro and in vivo, indicating that RAV1 directly down-regulates the expression of ABI3, ABI4, and ABI5.